However, in the olfactory bulb of adult zebrafish, gfap is expressed in processes. Labome lists 954119 antibody products against 88487 genes, including 19044 human, 15677 mouse, and 14548 rat genes. Gfap antibody fitc is a rabbit polyclonal antibody against gfap conjugated to fitc. Gfap antibody gf5 astrocyte marker ab10062 immunocytochemical detection in stimulated cor1 cells.
Zebrafish astroglial morphology in the olfactory bulb is. This gfap works for immunohistochemistry, immunocytochemistry. Thermo scientific lab vision gfap glial fibrillary acidic. Zebra fish is a good animal model for toxicity te sting. Antigfap, gfap antibody sigma antiglial fibrillary. Antiglial fibrillary acidic protein antibody antigfap. A monoclonal antibody stains radial glia in the adult zebrafish. We appreciate as much detail as possible and references as appropriate. Utilizing bac transgenesis, we generated transgenic zebrafish in. Molecular characterization of retinal stem cells and their niches in. Using the am41tgfbw on a zebrafish with gfp green fluorescent proteins. Zebrafish have astrocytes 23, and zebrafish gfap shares 67% identity and 77%.
In 2 dpf embryos, gfapdriven green fluorescent protein gfp expression was detectable in most parts of the brain, retina, lens and cells of the spinal cord fig. Recombinant human gfapgfap, a classiii intermediate filament, is a cellspecific marker that, during the. Regulatory elements that direct the glialspecific expression of zebrafish gfap were identified in genomic sequences from the zebrafish gfap gene. Aggregationprone gfap mutation in alexander disease validated. Widely used and referenced in customer publications. We also describe a novel specific marker for muller glia, apoe. Immunofluorescent analysis of gfap in human ipscderived astrocytes.
I am interested in native or recombinant gfap protein without gst tag or in urea buffer. Compare antizebrafishfish antibody products from leading suppliers on biocompare. Gtx128741 wb image gfap antibody detects gfap protein by western blot analysis. Amount and localization of acetylated tubulin in zebrafish. A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid. Stimulated cor1 cells were fixed in formaldehyde, permeabilized, blocked in 1% bsa for 10 mins. Genetex currently offers a continuously expanding catalog of reagents designed expressly for zebrafish research, with more than 400 newly developed zebrafish antibodies. About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al.
A monoclonal antibody stains radial glia in the adult zebrafish danio rerio cns. G9269 sigmaaldrich antiglial fibrillary acidic protein antibody produced in rabbit igg fraction of antiserum, buffered aqueous solution synonym. Zebra fish with gfp green fluorescent proteins youtube. Please acknowledge the principal investigator, cite the article in which the plasmids were described, and include addgene in the materials and methods of. Novel hypophysiotropic agrp2 neurons and pineal cells revealed. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. We have generated transgenic zebrafish that express green fluorescent protein gfp in glial cells driven by the zebrafish glial fibrillary acidic protein gfap regulatory elements. Due to a lack of a working antibody against zebrafish nestin and to assess the degree of correlation between tg.
The 5th edition is available in print and within the zfin protocol wiki. The zebra fish, danio rerio, is a popular model organism used for in vivo experiments and studies in cell biology. This antibody reacts with human, mouse, rat, zebrafish samples. Abcam antibodies and reagents supplier, find any antibody. The cells were fixed with 4% paraformaldehyde for 15 min at room temperature, and then permeabilized and blocked with 0. Host rabbit type primary clonality polyclonal conjugate unconjugated target glial fibrillary acidic protein uniprot p146. Gfppositive cells exhibited a round cell body and long processes, similar to the morphology described for glial cells in zebrafish larvae bernardos and raymond, 2006. These antibodies can be used for applications including flow cytometry, western blotting, immunofluorescence.
Using zebra fish, this sbir proposes to develop a rapid in vivo assay for assessing drug induced neurotoxicity. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra. Ensure accurate, reproducible results in immunohistochemistry experiments with thermo scientific gfap glial fibrillary acidic protein, rabbit polyclonal antibody. This material is from the 4th edition of the zebrafish book. Alexander disease axd is an astrogliopathy that predominantly affects the white matter of the central nervous system cns, and is caused by a mutation in the gene encoding the glial. We employed two novel transgenic zebrafish lines and endogenous markers. Antibodies against gfap and gs were used to identify astroglial. Neither the content nor the benchsci technology and processes for selection have been evaluated by us. Our gfap antibodies can be used in a variety of model species. Clicking the images or links will redirect you to a website hosted by benchsci that provides thirdparty scientific content. Ensure accurate, reproducible results in immunohistochemistry, western blotting and immunofluorescence experiments with thermo scientific gfap glial fibrillary acidic protein. Gfappositive radial glial cells are an essential progenitor. Which is the best antibody to label gfap mouse or goat on ihcfluorescence brand and catalog number. Amount and localization of acetylated tubulin in zebrafish embryosintroduction.
The distribution of gfap immunoreactivity implies that enteric glia are. Our range of antibodies that recognize fish antigens come in a variety of formats. Glial fibrillary acidic protein gfap is a member of the class iii intermediate filament protein family. Please use this form to recommend updates to the information in zfin. Our data show that the zebrafish retina has two kinds of specialized niches that. This is a summary of zebrafish antibody products in labome database. Markers and controls zebrafish antibodies sigmaaldrich.
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